a mixed culture of 3 bacteria
. Assume that
Escherichia coli can
sucrose and lactose and also produce
carbon dioxide gas on fermenting these. Assume that
ferment only sucrose and ca
nnot ferment glucose
and lactose. Also on
fermenting sucrose it produces hydrogen sulphide gas.
ferment any of these 4 sugars
but forms endospores
and is sensitive to penicillin
they all give similar looking white
on an agar plate
pure cultures of these 3 bacteria using the following tools that you
learned in the lab.
(some or all)
of these tools
to generate pure cultures as
well as confirm them.
) Special media plates
McConkey Agar or Blood Agar or
Mannitol Agar plates)
Fermentation media tubes
Triple Sugar Iron (TSI)
or Durham tubes)
Any of the disc diffusion test
Assume for your answers the u
se of Agar plates or burners or boiling water bath
any reagent can be supplied
the names of bacteria using the rules of
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